In a qua­li­ta­ti­ve stu­dy of 161 GC-com­pa­ti­ble pe­sti­ci­des, the ma­jo­ri­ty for­med do­mi­nant LC-MS-like ions with li­mi­t­ed frag­men­ta­ti­on.

The re­sul­ting spec­tra enable re­lia­ble pre­cur­sor sel­ec­tion for MS2 ac­qui­si­ti­on.
Data-de­pen­dent MS2 spec­tra ge­ne­ra­ted from GC-SICRIT® were suc­cessful­ly matched against an LC-MS/MS pe­sti­ci­de li­bra­ry.

Ex­am­p­le: Dia­zi­non shows a do­mi­nant [M+H]+ ion un­der SICRIT®, en­ab­ling con­fi­dent MS2 ac­qui­si­ti­on and suc­cessful LC-li­bra­ry con­fir­ma­ti­on.

GC com­pounds be­co­me LC-li­bra­ry com­pa­ti­ble.

Quan­ti­ta­ti­ve per­for­mance was eva­lua­ted for 74 pe­sti­ci­des in an un­t­ar­ge­ted full scan MS1 ex­pe­ri­ment wi­t­hout sam­ple ex­tra­c­tion or en­rich­ment. Li­mits of de­tec­tion were cal­cu­la­ted using the EU spik­ed blank equa­ti­on, with 10 ppb de­fi­ned as the re­gu­la­to­ry bench­mark.

All but four com­pounds show­ed LOD va­lues be­low 10 ppb. Many com­pounds were de­tec­ted in the sub ppb ran­ge, and se­ve­ral re­a­ched the ppt le­vel. For ex­am­p­le, Fol­pet achie­ved an LOD of 5 ppb, well be­low its EU ma­xi­mum re­si­due li­mit of 30 to 70 ppb.

GC-SICRIT®-MS enhan­ces pe­sti­ci­de ana­ly­sis by shif­ting the fo­cus from frag­men­ta­ti­on pat­terns to struc­tu­ral­ly in­for­ma­ti­ve mole­cu­lar and qua­si mole­cu­lar ions. This en­ables con­fi­dent pre­cur­sor sel­ec­tion, MS/MS con­fir­ma­ti­on, and di­rect use of LC-MS/MS spec­tral li­bra­ri­es wi­thin a GC work­flow.

• Re­lia­ble mole­cu­lar ion de­tec­tion
• MS/MS ba­sed con­fir­ma­ti­on di­rect­ly from GC
• Ac­cess to es­tab­lished LC-MS/MS li­bra­ri­es
• Sen­si­ti­vi­ty ali­gned with re­gu­la­to­ry re­qui­re­ments
• Sui­ta­bi­li­ty for both tar­ge­ted and un­t­ar­ge­ted me­thods

The re­sult is a more in­for­ma­ti­ve and con­fir­ma­ti­on dri­ven GC-MS ap­proach wi­t­hout com­pro­mi­sing ana­ly­ti­cal per­for­mance.

In­te­res­ted in eva­lua­ting GC-SICRIT® for your pe­sti­ci­de work­flows?