Li­quid Chro­ma­to

Ge­ne­ral­ly, SICRIT is a gas pha­se tech­ni­que. By va­po­ri­zing the li­quid (so­lu­ti­on) in a de­di­ca­ted mo­du­le, SICRIT also en­ables to cou­ple with li­quid chro­ma­to­gra­phy (LC) or su­per­cri­ti­cal flu­id chro­ma­to­gra­phy (SFC), though.

Ex­em­pla­ry ana­ly­sis

Pro­vi­ding clea­ner spec­tra, hig­her salt to­le­rance, uni­ver­sal sol­vent com­pa­ti­bi­li­ty, and broa­der co­vera­ge of io­niza­ti­on.

The SICRIT Io­niza­ti­on Source is in­her­ent­ly a gas pha­se tech­ni­que that al­lows for the soft io­niza­ti­on of com­pounds that ran­ge in both sta­bi­li­ty and po­la­ri­ty, pro­vi­ding a clea­ner MS1 spec­tra than other io­niza­ti­on tech­ni­ques. Ad­di­tio­nal­ly, the LC Mo­du­le is de­si­gned to wi­th­stand hig­her li­quid flow ra­tes, com­pa­ti­bi­li­ty with com­mon ESI buf­fers and num­e­rous com­bi­na­ti­ons of sol­vent me­thods. By cou­pling the­se tech­no­lo­gies, we have ac­cess to a who­le new world of LC Chro­ma­to­gra­phy ap­pli­ca­ti­ons

Here, an MS1 spec­tra of Si­ma­zi­ne, a com­mon pe­sti­ci­de, can be ob­ser­ved from both ESI and SICRIT. Whe­re the SICRIT is able to pro­vi­de a less frag­men­ted, clea­rer MS1 spec­tra uti­li­zing the LC Mo­du­le.

Graphic about LC-MS Coupling that shows the cleaner MS1 spectrum of the observed pesticide Si­ma­zi­ne when using SICRIT compared to ESI.
Graphic that shows how LC-SICRIT®- LC-MS Coupling makes so far not io­nizable PFCs observable

When we com­bi­ne the func­tion­a­li­ty of LC Chro­ma­to­gra­phy using the LC Mo­du­le and SICRIT Io­niza­ti­on tech­no­lo­gy, we ex­pand the ran­ge of io­nizable com­pounds, many of which were pre­vious­ly un­available th­rough LC Chro­ma­to­gra­phy.

Per-Fluo­ro­na­ted Com­pounds (PFCs) are ex­tre­me­ly to­xic and of gre­at im­portance in both re­se­arch and in­dus­tri­al ap­pli­ca­ti­ons, the­r­e­fo­re, it is im­pe­ra­ti­ve that we can de­tect the­se mole­cu­les. With the SICRIT io­niza­ti­on tech­no­lo­gy we are able to not only de­tect the­se com­pounds ma­nu­al­ly, but can use the na­ti­ve com­pound iden­ti­fi­ca­ti­on ven­dor soft­ware to con­firm our fin­dings. Curr­ent­ly, non-po­lar PFCs are not io­nizable by con­ven­tio­nal LC-MS me­thods.

By ad­ding the ca­pa­bi­li­ty of li­quid chro­ma­to­gra­phy to the SICRIT tech­no­lo­gy, we can pro­vi­de sharp, clear peaks of io­ni­zed com­pon­ents. This al­lows us to ef­fec­tively ana­ly­ze com­plex mix­tures with ease.

Here, we pro­vi­de a set of about 200 uni­que pe­sti­ci­des pre­sen­ted in a rou­ti­ne LC-MS run. The­se com­pounds ran­ge in func­tion­a­li­ty and po­la­ri­ty and have been com­bi­ned into one lar­ge mix­tu­re, pro­du­cing di­stin­gu­is­ha­ble chro­ma­to­gram peaks, de­spi­te the vo­lu­me of ana­lytes.

Graphic that shows how LC-SICRIT®- LC-MS Coupling allows clear peaks in a rou­ti­ne LC-MS run of 200 uni­que pe­sti­ci­des

Re­la­ted App No­tes and Pro­duct No­tes

LC-SICRIT®-HRMS Ana­ly­sis of Non-Po­lar Li­pids
In this set of me­a­su­re­ments, we de­mons­tra­te the de­tec­tion ca­pa­bi­li­ties of 8 dif­fe­rent Tria­cyl-gly­ce­ri­des of va­ry­ing length and sa­tu­ra­ti­on with LC-SICRIT®-HRMS.

The SICRIT® LC/SFC Mo­du­le com­bi­nes the SICRIT® io­niza­ti­on tech­no­lo­gy with clas­si­cal sam­ple se­pa­ra­ti­on of li­quid or su­per­cri­ti­cal flu­id chro­ma­to­gra­phy

Dis­co­ver our Pro­ducts

Find the per­fect so­lu­ti­on for your use case, with Plasmion’s ad­van­ced tech­no­lo­gy and ex­per­ti­se in che­mi­cal ana­ly­sis for re­se­arch or in­dus­try.