Li­quid Chro­ma­to
gra­phy

Ge­ne­ral­ly, SICRIT® is a gas pha­se tech­ni­que. By va­po­ri­zing the li­quid (so­lu­ti­on) in a de­di­ca­ted mo­du­le, SICRIT® also en­ables to cou­ple with li­quid chro­ma­to­gra­phy (LC) or su­per­cri­ti­cal flu­id chro­ma­to­gra­phy (SFC), though.

Ex­em­pla­ry ana­ly­sis
re­sults

SICRIT® en­ables clea­ner spec­tra, hig­her salt to­le­rance, uni­ver­sal sol­vent com­pa­ti­bi­li­ty, and broa­der co­vera­ge of io­niza­ti­on for li­quid chro­ma­to­gra­phy.

The SICRIT® Ion Source is in­her­ent­ly a gas pha­se tech­ni­que that al­lows for the soft io­niza­ti­on of com­pounds that ran­ge in both sta­bi­li­ty and po­la­ri­ty, pro­vi­ding a clea­ner MS1 spec­tra than other io­niza­ti­on tech­ni­ques. Ad­di­tio­nal­ly, the LC Mo­du­le is de­si­gned to wi­th­stand hig­her li­quid flow ra­tes, com­pa­ti­bi­li­ty with com­mon ESI buf­fers and num­e­rous com­bi­na­ti­ons of sol­vent me­thods. By cou­pling the­se tech­no­lo­gies, we have ac­cess to a who­le new world of li­quid chro­ma­to­gra­phy ap­pli­ca­ti­ons

Here, an MS1 spec­tra of Si­ma­zi­ne, a com­mon pe­sti­ci­de, can be ob­ser­ved from both ESI and SICRIT®. Whe­re the SICRIT® is able to pro­vi­de a less frag­men­ted, clea­rer MS1 spec­tra uti­li­zing the LC Mo­du­le.

Graphic about LC-MS Coupling that shows the cleaner MS1 spectrum of the observed pesticide Si­ma­zi­ne when using SICRIT compared to ESI.
Graphic that shows how LC-SICRIT®- LC-MS Coupling makes so far not io­nizable PFCs observable

When we com­bi­ne the func­tion­a­li­ty of li­quid chro­ma­to­gra­phy using the LC Mo­du­le and SICRIT Io­niza­ti­on Tech­no­lo­gy, we ex­pand the ran­ge of io­nizable com­pounds, many of which were pre­vious­ly un­available th­rough LC Chro­ma­to­gra­phy.

Per-Fluo­ro­na­ted Com­pounds (PFCs) are ex­tre­me­ly to­xic and of gre­at im­portance in both re­se­arch and in­dus­tri­al ap­pli­ca­ti­ons, the­r­e­fo­re, it is im­pe­ra­ti­ve that we can de­tect the­se mole­cu­les. With the SICRIT® Io­niza­ti­on Tech­no­lo­gy we are able to not only de­tect the­se com­pounds ma­nu­al­ly, but can use the na­ti­ve com­pound iden­ti­fi­ca­ti­on ven­dor soft­ware to con­firm our fin­dings. Curr­ent­ly, non-po­lar PFCs are not io­nizable by con­ven­tio­nal LC-MS me­thods.

By ad­ding the ca­pa­bi­li­ty of li­quid chro­ma­to­gra­phy to the SICRIT® Io­niz­a­ton Tech­no­lo­gy, we can pro­vi­de sharp, clear peaks of io­ni­zed com­pon­ents. This al­lows us to ef­fec­tively ana­ly­ze com­plex mix­tures with ease.

Here, we pro­vi­de a set of about 200 uni­que pe­sti­ci­des pre­sen­ted in a rou­ti­ne LC-MS run. The­se com­pounds ran­ge in func­tion­a­li­ty and po­la­ri­ty and have been com­bi­ned into one lar­ge mix­tu­re, pro­du­cing di­stin­gu­is­ha­ble chro­ma­to­gram peaks, de­spi­te the vo­lu­me of ana­lytes.

Graphic that shows how LC-SICRIT®- LC-MS Coupling allows clear peaks in a rou­ti­ne LC-MS run of 200 uni­que pe­sti­ci­des

Know­ledge Base

Li­pi­do­mics De­coded: Tar­ge­ted As­sign­ment of Po­lar Li­pids using the SICRIT® LC-Mo­­du­­le
This stu­dy shows that by using the SICRIT® Io­niza­ti­on tech­ni­que, you can con­duct suc­cessful ana­ly­sis of a wide ran­ge of po­lar li­pids. Ad­di­tio­nal­ly, you can ob­tain a usable MS2 spec­tra that can be used for eit­her tar­ge­ted or un­t­ar­ge­ted ana­ly­sis.

Swit­ching io­niza­ti­on? No Pro­blem! Ana­ly­sis of 78 pe­sti­ci­des in com­plex ma­tri­ces using a stan­dard LC-ESI work­flow with SICRIT®
In this stu­dy, we uti­li­ze a HRMS cou­pled to the SICRIT® LC-Mo­­du­­le to ana­ly­ze 78 pe­sti­ci­des across mul­ti­ple clas­ses when ex­po­sed to se­ve­ral com­plex bio­lo­gi­cal ma­tri­ces to see how well we stand in mi­mi­cking the ESI func­tion­a­li­ty and io­niza­ti­on with an ESI spe­ci­fic me­thod, with ESI do­mi­na­ted ana­lytes of choice.

Dis­co­ver our Pro­ducts

Find the per­fect so­lu­ti­on for your use case, with Plasmion’s ad­van­ced tech­no­lo­gy and ex­per­ti­se in che­mi­cal ana­ly­sis for re­se­arch or in­dus­try.

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