In this stu­dy, we uti­li­ze a HRMS cou­pled to the SICRIT® LC-Mo­­du­­le to ana­ly­ze 78 pe­sti­ci­des across mul­ti­ple clas­ses when ex­po­sed to se­ve­ral com­plex bio­lo­gi­cal ma­tri­ces to see how well we stand in mi­mi­cking the ESI func­tion­a­li­ty and io­niza­ti­on with an ESI spe­ci­fic me­thod, with ESI do­mi­na­ted ana­lytes of choice.