MS
Ima­ging

Mass spec­tro­me­try (MS) ima­ging vi­sua­li­zes the spa­ti­al ar­ran­ge­ment of mole­cu­les, such as bio­mar­kers, me­ta­boli­tes, pep­ti­des, or pro­te­ins, ba­sed on their mole­cu­lar mas­ses. The SICRIT® Ion Source in com­bi­na­ti­on with a la­ser ab­la­ti­on set­up or as post-io­niza­ti­on de­vice for (AP-) MALDI en­ables MS ima­ging down to 5μm.

The SICRIT® Ion Source in com­bi­na­ti­on with a la­ser ab­la­ti­on set­up or as post-io­niza­ti­on de­vice for (AP-) MALDI en­ables MS ima­ging down to 5μm.

Icon in a green hexagon that is marked as number one that resembles a laser beam hitting a particle

La­ser-
Ab­la­ti­on

Icon in a green hexagon that is marked as number two resembling a laser beam hitting a highlighted particle

Pos­tio­niza­ti­on
for (AP)MALDI

High Re­so­lu­ti­on La­ser Ab­la­ti­on

Graphic that shows the com­bi­na­ti­on of a UV-la­ser ab­la­ti­on sys­tem with a heated transfer line and the flow-th­rough SICRIT® Ion Source to a MS

The com­bi­na­ti­on of a UV-la­ser ab­la­ti­on sys­tem with the flow-th­rough SICRIT® Ion Source al­lows for a ef­fi­ci­ent, soft io­niza­ti­on-ba­sed vi­sua­liza­ti­on of mole­cu­les with a very high spa­ti­al re­so­lu­ti­on.

The SICRIT® Ion Source can be hy­phen­a­ted to any com­mer­cial la­ser ab­la­ti­on sys­tem and ne­ar­ly any API mass spec­tro­me­ter that is com­mer­ci­al­ly available. This makes this tech­ni­que easy to ad­apt to in­di­vi­du­al needs re­gar­ding the sam­ple type and ana­ly­ti­cal ques­ti­on.“

Elia, E.A. et al, Anal. Chem. 2020, 92, 15285–15290

Pos­tio­niza­ti­on For (AP)Maldi

Graphic that shows the com­bi­na­ti­on of a UV-la­ser ab­la­ti­on sys­tem as a primary ionization method coupled via a heated transfer line to the flow-th­rough SICRIT® Ion Source as a secondary ionization to a MS

Using the flow-th­rough SICRIT® Ion Source as post-io­niza­ti­on de­vice for AP-MALDI ima­ging al­lows to io­ni­ze and vi­sua­li­ze ad­di­tio­nal mole­cu­les that have not been io­ni­zed with the pri­ma­ry io­niza­ti­on source. This si­gni­fi­cant­ly ex­pands the mass spec­tro­me­tric view on the sam­ple in an MS ima­ging ap­proach.

The ease with which the SICRIT® de­vice can be in­stal­led and the mi­ni­mal need for op­ti­miza­ti­on pres­ents this com­mer­ci­al­ly available tool as an at­trac­ti­ve me­thod for simp­le post-io­niza­ti­on for any AP-MALDI MS ima­ging.“

Elia, E.A. et al, Anal. Chem. 2020, 92, 15285–15290

Know­ledge Base

Sub­cel­lu­lar MSI of li­pids and nu­cleo­ti­des using trans­mis­si­on geo­me­try am­bi­ent la­ser de­sorp­ti­on and SICRIT® io­niza­ti­on
A plas­ma-as­sis­ted trans­mis­si­on-geo­me­try la­ser de­sorp­ti­on me­thod en­ables sub­cel­lu­lar MS ima­ging of li­pids and nu­cleo­ti­des at 1 µm re­so­lu­ti­on, enhan­cing io­niza­ti­on ef­fi­ci­en­cy and reve­al­ing spa­ti­al mole­cu­lar dis­tri­bu­ti­ons wi­thin tis­sues.

Mul­ti­ple­xed Tu­mor Mar­ker De­tec­tion via La­ser Ab­la­ti­on-DBDI-MS
This stu­dy de­mons­tra­tes la­ser ablation-SICRIT®-MS for mul­ti­ple­xed de­tec­tion of tu­mor bio­mar­kers, achie­ving high sen­si­ti­vi­ty and ef­fi­ci­ent quan­ti­ta­ti­on from mi­ni­mal se­rum and cel­lu­lar samples.

Dis­co­ver our Pro­ducts

Find the per­fect so­lu­ti­on for your use case, with Plasmion’s ad­van­ced tech­no­lo­gy and ex­per­ti­se in che­mi­cal ana­ly­sis for re­se­arch or in­dus­try.

Dis­co­ver